Journal: PLoS ONE

Article Title: Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics

doi: 10.1371/journal.pone.0217941

Figure Lengend Snippet: At 15dpc (A) is present in the nucleus of the (arrowheads), whereas DAZL (D) is localized in the cytoplasm, (arrowheads). At 16dpc (B and E) both proteins are localized in the cytoplasm (arrowheads), but PIWIL4 distribution is more restricted to the region close to the nucleus (arrowheads), whereas DAZL is diffusely distributed throughout the cytoplasm (arrowhead). At 19dpc (C and F) , as observed for MAEL and TDRD9, the nuage (arrowheads) is is adjacent to a restricted portion of the gonocyte nucleus.

Article Snippet: The membranes were incubated with blocking buffer (PBS; 0.1% Tween-20; 5% milk) at 37°C and then with the antibodies anti-DAZL (1:400), anti-MAEL (1:400), anti-PIWI4 (1:200) or anti-TDRD9 (1:300) for 16h. β-Actin (1:100; Cell Signalling, #4970) was used as endogenous control.

Techniques:

Journal: PLoS ONE

Article Title: Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics

doi: 10.1371/journal.pone.0217941

Figure Lengend Snippet: A) DAZL, that has been used as the bait, was present in the whole gonad extract (E19) and is absent from the Co-IP yield (Co-IP). B) PIWIL4 was detected in the Co-IP yield (Co-IP) and in the whole gonads extract (E19). C) TDRD9 was detected in the whole extract (E19), but not in the Co-IP yield (Co-IP). D) MAEL, as observed for PIWIL4, was detected in the whole extract and in the Co-IP yield (Co-IP). E) ß-Actin was used as the reference protein. T: testis; M: muscle.

Article Snippet: The membranes were incubated with blocking buffer (PBS; 0.1% Tween-20; 5% milk) at 37°C and then with the antibodies anti-DAZL (1:400), anti-MAEL (1:400), anti-PIWI4 (1:200) or anti-TDRD9 (1:300) for 16h. β-Actin (1:100; Cell Signalling, #4970) was used as endogenous control.

Techniques: Co-Immunoprecipitation Assay

Journal: Journal of ovarian research

Article Title: Maelstrom promotes tumor metastasis through regulation of FGFR4 and epithelial-mesenchymal transition in epithelial ovarian cancer.

doi: 10.1186/s13048-022-00992-4

Figure Lengend Snippet: Fig. 3 MAEL induces EMT and up-regulates FGFR4 expression. Western blot analysis showing that the expression of α-catenin, β-catenin and E-cadherin increased while the expression of vimentin and fibronectin decreased in MAEL-silenced ES2 cells, compared to those in corresponding control cells. The expression of α-catenin, β-catenin and E-cadherin decreased while the expression of vimentin and fibronectin increased in stable MAEL-transfected HO8910 cells compared to those in corresponding control cells. B Nine genes (i.e. SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3 and FGFR4) were found to exhibit a more than 2-fold different expression in ES2-shMAEL-1 cells compared to that in ES2-shControl cells using a Human Tumor Metastasis RT2 Profiler™ PCR Array. C The expression of SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3 and FGFR4 was verified in ES2-shMAEL-1 cells and their respective controls by western blot. D Overexpression of MAEL and FGFR4 were detected by IHC in a EOC case (200×)

Article Snippet: Equal amounts of proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Danvers, MA, USA), followed by incubation overnight at 4 °C with primary antibodies against the following human proteins: MAEL (Sigma, USA; 1:1000 dilution), α-catenin, β-catenin, Vimentin, E-cadherin, Fibronectin (all from BD Transduction Laboratories, Franklin Lakes, NJ; 1:1000 dilution), SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3, FGFR4 (all from Cell Signaling Technology; 1:1000 dilution), and GAPDH (Proteintech; 1:5000 dilution).

Techniques: Expressing, Western Blot, Control, Transfection, Over Expression

Journal: Journal of ovarian research

Article Title: Maelstrom promotes tumor metastasis through regulation of FGFR4 and epithelial-mesenchymal transition in epithelial ovarian cancer.

doi: 10.1186/s13048-022-00992-4

Figure Lengend Snippet: Fig. 3 MAEL induces EMT and up-regulates FGFR4 expression. Western blot analysis showing that the expression of α-catenin, β-catenin and E-cadherin increased while the expression of vimentin and fibronectin decreased in MAEL-silenced ES2 cells, compared to those in corresponding control cells. The expression of α-catenin, β-catenin and E-cadherin decreased while the expression of vimentin and fibronectin increased in stable MAEL-transfected HO8910 cells compared to those in corresponding control cells. B Nine genes (i.e. SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3 and FGFR4) were found to exhibit a more than 2-fold different expression in ES2-shMAEL-1 cells compared to that in ES2-shControl cells using a Human Tumor Metastasis RT2 Profiler™ PCR Array. C The expression of SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3 and FGFR4 was verified in ES2-shMAEL-1 cells and their respective controls by western blot. D Overexpression of MAEL and FGFR4 were detected by IHC in a EOC case (200×)

Article Snippet: Equal amounts of proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Danvers, MA, USA), followed by incubation overnight at 4 °C with primary antibodies against the following human proteins: MAEL (Sigma, USA; 1:1000 dilution), α-catenin, β-catenin, Vimentin, E-cadherin, Fibronectin (all from BD Transduction Laboratories, Franklin Lakes, NJ; 1:1000 dilution), SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3, FGFR4 (all from Cell Signaling Technology; 1:1000 dilution), and GAPDH (Proteintech; 1:5000 dilution).

Techniques: Expressing, Western Blot, Control, Transfection, Over Expression